Western Blot analysis of RFFL expression in transfected 293T cell line by RFFL monoclonal antibody. Lane 1: RFFL transfected lysate (36.6kD). Lane 2: Non-transfected lysate.

Detection limit for recombinant GST tagged RFFL is 0.03ng/ml as a capture antibody.

Western blot analysis of RFFL over-expressed 293 cell line, cotransfected with RFFL Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with RFFL monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.